CBBM Lecture "Sleep-wake cycles drive daily dynamics of phosphorylation in synapses" by


Prof. Dr. Charo Robles, Systems Chronobiology-Robles Lab, Institute of Medical Psychology, Ludwig-Maximilians-Universität München


will take place on Tuesday, April 9, 2019 from 17:15 to 18:15 hours in CBBM, Ground Floor, Seminar Room B1/B2.

Host: Prof. Dr. Henrik Oster
Institute of Neurobiology
University of Lübeck


Abstract

Mass-spectrometry (MS) based quantitative proteomics is a powerful technology used to study tissue, cellular and sub-cellular proteomes as well as protein post-translational modifications (PTMs). This technology has revolutionized the quantification of protein levels and modifications for in many biological fields, amongst them chronobiology. In this talk I will present our work in the last years pioneering the use of MS-based quantitative proteomics to study circadian protein function in the liver by means of protein abundance and phosphorylation dependent protein function. In addition, I will present our latest work studying dynamics of proteome and phosphoproteome in isolated synaptoneurosomes from mouse forebrain in normal and also under sleep deprivation conditions.


Biosketch

Charo Robles studied Biology at the University of Leon (Spain). She moved to Madrid (Universidad Autonoma) to do her doctorate degree under the supervision of Carlos Martinez studying apoptosis in the immune system. After that she moved to Harvard Medical School as a postdoctoral fellow in the laboratory of Charles Weitz where she established the first ex vivo system to identify core circadian clock components by affinity purification-mass spectrometry. She then joined the laboratory of Matthias Mann with a Marie Curie Grant to pioneer the application of quantitative proteomics to the circadian field; describing, for the first time, endogenous daily rhythms of proteome and phosphorylation in total mouse livers as well as in isolated liver mitochondria. Since April 2017 Charo is a Tenure Track Professor at the LMU Munich. Her research group has recently purchased a state-of-the-art instrument (Q-Exactive™ HF-X) purchased to continue using mass spectrometry-based proteomics to study basic circadian molecular mechanism as well as post-translational mechanism regulating circadian metabolism and physiology.